Calculate percent cell viability from MTT assay absorbance data, normalize to controls, and determine IC50 of cytotoxic compounds.
| Constant | Symbol | Value |
|---|---|---|
| Speed of light | c | 2.99792458×10⁸ |
| Planck's constant | h | 6.62607015×10⁻³‴ |
| Boltzmann constant | kʙ | 1.380649×10⁻²³ |
| Avogadro's number | Nₐ | 6.02214076×10²³ |
| Gravitational constant | G | 6.6743×10⁻¹¹ |
| Gas constant | R | 8.31446 |
| Elementary charge | e | 1.602176634×10⁻¹⁹ |
| Electron mass | mₑ | 9.1093837015×10⁻³¹ |
| Proton mass | mₚ | 1.67262192369×10⁻²⁷ |
| Fine-structure constant | α | 7.2973525693×10⁻³ |
Calculate percent cell viability from MTT assay absorbance data, normalize to controls, and determine IC50 of cytotoxic compounds
Each component has a specific meaning:
Note: Interpret the mtt assay result against the clinical thresholds and context described above.
Enter the MTT assay absorbance data, normalize for the patient or scenario you are assessing. Calculate percent cell viability from MTT assay absorbance data, normalize to controls, and determine IC50 of cytotoxic compounds. Use the mtt assay result to inform your clinical assessment.