Calculate optical microscopy resolution limit using Rayleigh criterion: d = 1.22 * lambda / (NA_obj + NA_cond) for transmitted and epi-fluorescence.
| Constant | Symbol | Value |
|---|---|---|
| Speed of light | c | 2.99792458×10⁸ |
| Planck's constant | h | 6.62607015×10⁻³‴ |
| Boltzmann constant | kʙ | 1.380649×10⁻²³ |
| Avogadro's number | Nₐ | 6.02214076×10²³ |
| Gravitational constant | G | 6.6743×10⁻¹¹ |
| Gas constant | R | 8.31446 |
| Elementary charge | e | 1.602176634×10⁻¹⁹ |
| Electron mass | mₑ | 9.1093837015×10⁻³¹ |
| Proton mass | mₚ | 1.67262192369×10⁻²⁷ |
| Fine-structure constant | α | 7.2973525693×10⁻³ |
Calculate optical microscopy resolution limit using Rayleigh criterion: d = 1.22 * lambda / (NA_obj + NA_cond) for transmitted and epi-fluorescence
Each component has a specific meaning:
Note: Interpret the rayleigh resolution result against the clinical thresholds and context described above.
Enter the Rayleigh criterion: d = 1 for the patient or scenario you are assessing. Calculate optical microscopy resolution limit using Rayleigh criterion: d = 1.22 * lambda / (NA_obj + NA_cond) for transmitted and epi-fluorescence. Use the rayleigh resolution result to inform your clinical assessment.