Determine molar extinction coefficient from fluorescence excitation spectrum and relate quantum yield to fluorescence brightness.
| Constant | Symbol | Value |
|---|---|---|
| Speed of light | c | 2.99792458×10⁸ |
| Planck's constant | h | 6.62607015×10⁻³‴ |
| Boltzmann constant | kʙ | 1.380649×10⁻²³ |
| Avogadro's number | Nₐ | 6.02214076×10²³ |
| Gravitational constant | G | 6.6743×10⁻¹¹ |
| Gas constant | R | 8.31446 |
| Elementary charge | e | 1.602176634×10⁻¹⁹ |
| Electron mass | mₑ | 9.1093837015×10⁻³¹ |
| Proton mass | mₚ | 1.67262192369×10⁻²⁷ |
| Fine-structure constant | α | 7.2973525693×10⁻³ |
Determine molar extinction coefficient from fluorescence excitation spectrum and relate quantum yield to fluorescence brightness
Each component has a specific meaning:
Note: Interpret the extinction coefficient result against the clinical thresholds and context described above.
Enter the fluorescence excitation spectrum, relate quantum yield for the patient or scenario you are assessing. Determine molar extinction coefficient from fluorescence excitation spectrum and relate quantum yield to fluorescence brightness. Use the extinction coefficient result to inform your clinical assessment.