Calculate optimal PCR annealing temperature from primer Tm values and GC content for standard, touchdown, and gradient PCR protocols.
| Constant | Symbol | Value |
|---|---|---|
| Speed of light | c | 2.99792458×10⁸ |
| Planck's constant | h | 6.62607015×10⁻³‴ |
| Boltzmann constant | kʙ | 1.380649×10⁻²³ |
| Avogadro's number | Nₐ | 6.02214076×10²³ |
| Gravitational constant | G | 6.6743×10⁻¹¹ |
| Gas constant | R | 8.31446 |
| Elementary charge | e | 1.602176634×10⁻¹⁹ |
| Electron mass | mₑ | 9.1093837015×10⁻³¹ |
| Proton mass | mₚ | 1.67262192369×10⁻²⁷ |
| Fine-structure constant | α | 7.2973525693×10⁻³ |
Calculate optimal PCR annealing temperature from primer Tm values and GC content for standard, touchdown, and gradient PCR protocols
Each component has a specific meaning:
Note: Interpret the pcr annealing result against the clinical thresholds and context described above.
Enter the GC content for the patient or scenario you are assessing. Calculate optimal PCR annealing temperature from primer Tm values and GC content for standard, touchdown, and gradient PCR protocols. Use the pcr annealing result to inform your clinical assessment.