Estimate protein isoelectric point (pI) from amino acid sequence composition using Henderson-Hasselbalch equation and pKa values of ionizable residues.
| Constant | Symbol | Value |
|---|---|---|
| Speed of light | c | 2.99792458×10⁸ |
| Planck's constant | h | 6.62607015×10⁻³‴ |
| Boltzmann constant | kʙ | 1.380649×10⁻²³ |
| Avogadro's number | Nₐ | 6.02214076×10²³ |
| Gravitational constant | G | 6.6743×10⁻¹¹ |
| Gas constant | R | 8.31446 |
| Elementary charge | e | 1.602176634×10⁻¹⁹ |
| Electron mass | mₑ | 9.1093837015×10⁻³¹ |
| Proton mass | mₚ | 1.67262192369×10⁻²⁷ |
| Fine-structure constant | α | 7.2973525693×10⁻³ |
Estimate protein isoelectric point (pI) from amino acid sequence composition using Henderson-Hasselbalch equation and pKa values of ionizable residues
Each component has a specific meaning:
Note: Interpret the protein pi result against the clinical thresholds and context described above.
Enter the amino acid sequence composition for the patient or scenario you are assessing. Estimate protein isoelectric point (pI) from amino acid sequence composition using Henderson-Hasselbalch equation and pKa values of ionizable residues. Use the protein pi result to inform your clinical assessment.