Calculate protein thermal melting temperature Tm from differential scanning calorimetry or CD thermal denaturation data using two-state model.
| Constant | Symbol | Value |
|---|---|---|
| Speed of light | c | 2.99792458×10⁸ |
| Planck's constant | h | 6.62607015×10⁻³‴ |
| Boltzmann constant | kʙ | 1.380649×10⁻²³ |
| Avogadro's number | Nₐ | 6.02214076×10²³ |
| Gravitational constant | G | 6.6743×10⁻¹¹ |
| Gas constant | R | 8.31446 |
| Elementary charge | e | 1.602176634×10⁻¹⁹ |
| Electron mass | mₑ | 9.1093837015×10⁻³¹ |
| Proton mass | mₚ | 1.67262192369×10⁻²⁷ |
| Fine-structure constant | α | 7.2973525693×10⁻³ |
Calculate protein thermal melting temperature Tm from differential scanning calorimetry or CD thermal denaturation data using two-state model
Each component has a specific meaning:
Note: Interpret the protein tm result against the clinical thresholds and context described above.
Enter the differential scanning calorimetry or CD thermal denaturation data for the patient or scenario you are assessing. Calculate protein thermal melting temperature Tm from differential scanning calorimetry or CD thermal denaturation data using two-state model. Use the protein tm result to inform your clinical assessment.